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TAmiRNA – Triple A research and development of RNA diagnostics and therapeutics
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TAmiRNA – Introducing personalized medicine to Aging
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TAmiRNA – Harnessing the power of microRNAs for development of innovative diagnostic and therapeutic tools
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TAmiRNA – Helping to enhance your stability and quality of life with advanced age

our publications: 2016-2014


Original research article on discovery of novel microRNA biomarkers for the mangement of postmenopausal and diabetic osteoporosis.

Heilmeier U, Hackl M, Skalicky S, Weilner S, Schroeder F, Vierlinger K, et al. Serum microRNAs Are Indicative of Skeletal Fractures in Postmenopausal Women with and without Type 2 Diabetes and Influence Osteogenic and Adipogenic Differentiation of Adipose-Tissue Derived Mesenchymal Stem Cells In Vitro. J Bone Miner Res 2016. Dec;31(12):2173-2192

PubMed PMID: 27345526

Original research article on microRNAs and tissue homeostasis.

Kocijan R, Muschitz C, Geiger E, Skalicky S, Baierl A, Dormann R, et al. Circulating microRNA signatures in patients with idiopathic and postmenopausal osteoporosis and fragility fractures. J Clin Endocrinol Metab 2016:jc.2016-2365.

PubMed PMID: 27552543

Original research article investigating the donor-age dependent impact of extracellular vesicles on osteogenesis in vitro.

Weilner S, Keider V, Winter M, Harreither E, Salzer B, Weiss F, Schraml E, Messner P, Pietschmann P, Hildner F, Gabriel C, Redl H, Grillari-Voglauer R, Grillari J. Vesicular Galectin-3 levels decrease with donor age and contribute to the reduced osteo-inductive potential of human plasma derived extracellular vesicles. Aging (Albany NY). 2016 Jan 9.

PubMed PMID: 26752347


Original research article on urine derived mesenchymal stem cells and their capability to differentiate into osteoblasts, adipocytes and keratinocytes.

Schosserer M, Reynoso R, Wally V, Jug B, Kantner V, Weilner S, Buric I, Grillari J, Bauer JW, Grillari-Voglauer R. Urine is a novel source of autologous mesenchymal stem cells for patients with epidermolysis bullosa. BMC Res Notes. 2015 Dec 10;8(1):767. doi: 10.1186/s13104-015-1686-7.

PubMed PMID: 26654529


Review on microRNAs and their usability as biomarkers for bone diseases

Hackl M, Heilmeier U, Weilner S, Grillari J.  Circulating microRNAs as novel biomarkers for bone diseases - Complex signatures for multifactorial diseases? Mol Cell Endocrinol. 2015 Oct 23. pii: S0303-7207(15)30116-7. doi: 10.1016/j.mce.2015.10.015.

PubMed PMID: 26525415


Original research article which reports the identification of circulating microRNAs that are changed in the course of recent osteoporotic fractures in postmenopausal women, and which show osteogenic activity in vitro.

Weilner S, Skalicky S, Salzer B, Keider V, Wagner M, Hildner F, Gabriel C, Dovjak P, Pietschmann P, Grillari-Voglauer R, Grillari J, Hackl M.  Differentially circulating miRNAs after recent osteoporotic fractures can influence osteogenic differentiation. Bone. 2015 May 28;79:43-51.

PubMed PMID: 26026730


Orginial research article describing the expansion of the Chinese hamster ovary cell miRNome through massive-parallel sequencing and microarray analysis.

Diendorfer AB, Hackl M, Klanert G, Jadhav V, Reithofer M, Stiefel F, Hesse F, Grillari J, Borth N. Annotation of additional evolutionary conserved microRNAs in CHO cells from updated genomic data. Biotechnol Bioeng. 2015 Jul;112(7):1488-93.

PubMed PMID: 25689160 


Original research article on investigating in vitro shockwave treatment (IVSWT) effects on lymphatic endothelial cell (LEC) behavior and lymphangiogenesis.

Rohringer S, Holnthoner W, Hackl M, Weihs AM, Rünzler D, Skalicky S, Karbiener M, Scheideler M, Pröll J, Gabriel C, Schweighofer B, Gröger M, Spittler A, Grillari J, Redl H.  Molecular and cellular effects of in vitro shockwave treatment on lymphatic endothelial cells. PLoS One. 2014 Dec 11;9(12):e114806. doi: 10.1371/journal.pone.0114806.

PubMed PMID: 25502694.


Original research article on circulating microRNAs in chronic HIV-infected patients and elite control patients, and their potential use as therapeutic targets:

Reynoso R, Laufer N, Hackl M, Skalicky S, Monteforte R, Turk G, Carobene M, Quarleri J, Cahn P, Werner R, Stoiber H, Grillari-Voglauer R, Grillari J.  MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro (2014). Scientific Reports, Aug 1;4:5915.

PubMed PMID: 25081906.


Original research article reporting microarray data of microRNA and mRNA transcription in low and high producing CHO cell lines during a continuous pilot-scale fermentation process

Maccani A, Hackl M, Leitner C, Steinfellner W, Graf AB, Tatto NE, Karbiener M, Scheideler M, Grillari J, Mattanovich D, Kunert R, Borth N, Grabherr R, Ernst W.  Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation (2014). Appl Microbiol Biotechnol. Sep;98(17):7535-48. 

PubMed PMID: 25052466.


Original research article reporting the positive effect of an oncogenic microRNA – miR-17 – on recombinant protein expression in CHO cell lines

Jadhav V, Hackl M, Klanert G, Hernandez Bort JA, Kunert R, Grillari J, Borth N. Stable overexpression of miR-17 enhances recombinant protein production of CHO cells (2014). J Biotechnol. 175:38-44.

PubMed PMID: 24518263;


Original research article reporting the importance of post-transcriptional processing of microRNAs by DICER for CHO cell proliferation

Hackl M, Jadhav V, Klanert G, Karbiener M, Scheideler M, Grillari J, Borth N. Analysis of microRNA transcription and post-transcriptional processing by Dicer in the context of CHO cell proliferation (2014). J Biotechnol. pii:S0168-1656(14)00037-6.

PubMed PMID: 24486028.


Orginal research article reporting strategies for improved microRNA overexpression in CHO cell lines for cell engineering purposes

Klanert G, Jadhav V, Chanoumidou K, Grillari J, Borth N, Hackl M. Endogenous microRNA clusters outperform chimeric sequence clusters in Chinese hamster ovary cells (2014). Biotechnol J. 9(4):538-44.

PubMed PMID: 24323929.

Publications 2013 and earlier


Review of the importance of microRNAs in mammalian cell culture technology
Jadhav V, Hackl M, Druz A, Shridhar S, Chung CY, Heffner KM, Kreil DP, Betenbaugh M, Shiloach J, Barron N, Grillari J, Borth N. CHO microRNA engineering is growing up: recent successes and future challenges (2013). Biotechnol Adv. 31(8):1501-13. Review.  

PubMed PMID: 23916872

Review of the importance of secreted microRNAs during aging of cells, tissues and entire organisms.

Weilner, S., Schraml, E., Redl, H., Grillari-Voglauer, R., Grillari, J. Secretion of microvesicular miRNAs in cellular and organismal aging (2013) Experimental Gerontology, 48 (7), pp. 626-633.    

PubMed PMID:23283304

Original research article on the role of miR-21 in cellular lifespan
Dellago, H., Preschitz-Kammerhofer, B., Terlecki-Zaniewicz, L., Schreiner, C., Fortschegger, K., Chang, M.W.-F., Hackl, M., Monteforte, R., Kühnel, H., Schosserer, M., Gruber, F., Tschachler, E., Scheideler, M., Grillari-Voglauer, R., Grillari, J., Wieser, M. High levels of oncomiR-21 contribute to the senescence-induced growth arrest in normal human cells and its knock-down increases the replicative lifespan (2013) Aging Cell, 12 (3), pp. 446-458.
PubMed PMID: 23496142

Bioinformatic prediction of piRNAs – a new class of small non-coding RNAs – in a common recombinant therapeutic production cell line
Gerstl, M.P., Hackl, M., Graf, A.B., Borth, N., Grillari, J. Prediction of transcribed PIWI-interacting RNAs from CHO RNAseq data (2013) Journal of Biotechnology, 166 (1-2), pp. 51-57.   

PubMed PMID: 23639388

Original research article on the relevance of microRNAs in mediating the effect of UVB-irradiation on fibroblasts.
Greussing, R., Hackl, M., Charoentong, P., Pauck, A., Monteforte, R., Cavinato, M., Hofer, E., Scheideler, M., Neuhaus, M., Micutkova, L., Mueck, C., Trajanoski, Z., Grillari, J., Jansen-Dürr, P. Identification of microRNA-mRNA functional interactions in UVB-induced senescence of human diploid fibroblasts (2013) BMC Genomics, 2013 Apr 4;14:224.  
PubMed PMID: 23557329 



Protocol describing the isolation and subsequent reprogramming of urine cell into pluripotent stem cells.

Zhou, T., Benda, C., Dunzinger, S., Huang, Y., Ho, J.C., Yang, J., Wang, Y., Zhang, Y., Zhuang, Q., Li, Y., Bao, X., Tse, H.-F., Grillari, J., Grillari-Voglauer, R., Pei, D., Esteban, M.A. Generation of human induced pluripotent stem cells from urine samples (2012) Nature Protocols, 7 (12), pp. 2080-2089.  PubMed PMID: 23138349

Opinion Article on the use of microRNAs in engineering cell lines to become better tools for the production of recombinant therapeutic proteins
Hackl, M., Borth, N., Grillari, J. MiRNAs - pathway engineering of CHO cell factories that avoids translational burdening (2012) Trends in Biotechnology, 30 (8), pp. 405-406.   PubMed PMID:22673691

Orignial research article on a microRNA that controls DNA Damage in T-Cells in response to chemotherapeutic treatment with Etoposide
Brunner, S., Herndler-Brandstetter, D., Arnold, C.R., Wiegers, G.J., Villunger, A., Hackl, M., Grillari, J., Moreno-Villanueva, M., Bürkle, A., Grubeck-Loebenstein, B. Upregulation of miR-24 is associated with a decreased DNA damage response upon etoposide treatment in highly differentiated CD8 + T cells sensitizing them to apoptotic cell death (2012) Aging Cell, 11 (4), pp. 579-587.   PubMed PMID: 22435726

Original research article on microRNAs that control proliferation of CHO cell lines, which are the most frequently mammalian cell line for recombinant therapeutic protein production.
Jadhav, V., Hackl, M., Hernandez Bort, J.A., Wieser, M., Harreither, E., Kunert, R., Borth, N., Grillari, J. A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells (2012) Biotechnology and Bioengineering, 109 (6), pp. 1376-1385.     PubMed PMID: 22407745

Bioinformatic analysis of microRNA sequences and genomic organization in CHO cell lines.
Hackl, M., Jadhav, V., Jakobi, T., Rupp, O., Brinkrolf, K., Goesmann, A., Pühler, A., Noll, T., Borth, N., Grillari, J. Computational identification of microRNA gene loci and precursor microRNA sequences in CHO cell lines (2012) Journal of Biotechnology, 158 (3), pp. 151-155.   PubMed PMID: 22306111

Original research article describing  the changes in microRNA expression in vitro and ex vivo during aging.
Hackl, M., Brunner, S., Fortschegger, K., Schreiner, C., Micutkova, L., Mück, C., Laschober, G.T., Lepperdinger, G., Sampson, N., Berger, P., Herndler-Brandstetter, D., Wieser, M., Kühnel, H., Strasser, A., Rinnerthaler, M., Breitenbach, M., Mildner, M., Eckhart, L., Tschachler, E., Trost, A., Bauer, J.W., Papak, C., Trajanoski, Z., Scheideler, M., Grillari-Voglauer, R., Grubeck-Loebenstein, B., Jansen-Dürr, P., Grillari, J. miR-17, miR-19b, miR-20a, and miR-106a are down-regulated in human aging (2010) Aging Cell, 9 (2), pp. 291-296.


Opinion article on the potential of microRNAs for engineering the phenotype of cells to better fit to the needs in modern biotechnological processes.
Müller, D., Katinger, H., Grillari, J. MicroRNAs as targets for engineering of CHO cell factories (2008) Trends in Biotechnology, 26 (7), pp. 359-365.

Lepperdinger, G., Berger, P., Breitenbach, M., Frohlich, K.-U., Grillari, J., Grubeck-Loebenstein, B., Madeo, F., Minois, N., Zwerschke, W., Jansen-Durr, P. The use of genetically engineered model systems for research on human aging (2008) Frontiers in Bioscience, 13 (18), pp. 7022-7031.