Extracellular vesicle (EV) analysis

Comprehensive EVs characterization

Due to their capacity to transport proteins, lipids, as well as coding and non-coding RNAs, extracellular vesicles (EVs) play an important role in cellular communication. Therefore, EVs gain ever-increasing importance in the fields of regenerative and transplant medicine, anti-tumor therapy as well as immunotherapy. TAmiRNA provides services to characterize the DNA and RNA cargo of EVs using nucleic acid quantification, RT-qPCR, small RNAseq, and RNAseq. Our services are based on best practices released by the International Society for Extracellular Vesicles (ISEV).

Extracellular vesicle (EV) analysis
Extracellular vesicle (EV) analysis

benefits

Isolation of EV and protein fractions using size exclusion chromatography.

Nanoparticle Tracking Analysis (NTA) for quantitative and qualitative characterization as well as multiplex flow cytometry for analysis of exosome surface epitopes.

High sensitivity dsDNA and ssRNA quantification for characterization of nucleic acid content.

small RNAseq, RNAseq, or RT-qPCR for quantitative analysis of microRNA, mRNA and other ncRNA EV cargo.

service requirements

We can purify EVs (and protein fractions) from biofluids (serum, plasma, urine, etc.) and cell culture supernatant. Alternatively, we can process EV samples that have been generated externally. Get in touch with us to discuss your specific project details.

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Comprehensive EVs characterization

Comprehensive EVs characterization

reference projects

SVF-derived extracellular vesicles carry characteristic miRNAs in lipedema. 

Priglinger E., Strohmeier K., Weigl M et al. Sci Rep. 2020 Apr 29;10(1):7211.doi: 10.1038/s41598-020-64215-w.

MicroRNA-126 contributes to renal microvascular heterogeneity of VCAM-1 protein expression in acute inflammation

Asgeirsdóttir SA, van Solingen C, Kurniati NF et al. Am J Physiol Renal Physiol 2012 Jun 15;302(12):F1630-9. doi: 10.1152/ajprenal.00400.2011. Epub 2012 Mar 14.

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