microRNA Biomarkers of Liver Function and Disease
microRNA Biomarkers of Liver Function and Disease

Innovative blood-based liver biomarkers – hepatomiR®

The hepatomiR® test satisfies the urgent clinical need for an easily assessable preoperative test to predict liver function recovery after partial liver resection. This is critical to identify patients with high risk of postoperative liver dysfunction, and to plan according risk reduction measures.

The hepatomiR® test enables quantitative analysis of three liver-enriched microRNAs hsa-miR-122-5p, hsa-miR- 192-5p, and hsa-miR-151a-5p in plasma samples.  Our proprietary algorithm converts miRNA levels into a risk score that predicts postoperative liver dysfunction: Starlinger et al., HEPATOLOGY, Vol. 69, No. 6, 2019

Based on validated cut-off values for patient stratification, we have confirmed excellent prognostic performance in a cohort of HCC, CCC, and mCRC patients:

  • Positive predictive value (PPV): 83%
  • Negative predictive value (NPV): 85%
  • Odd’s Ratio: 19.7
microRNA Biomarkers of Liver Function and Disease

hepatomiR® RUO kit

microRNA Biomarkers of Liver Function and Disease

hepatomiR® CE-IVD kit

Product launch Spring 2022

hepatomiR® RUO kit

hepatomiR® CE-IVD kit

Product Launch Spring 2022

benefits

Simplicity: our optimized RT-qPCR chemistry ensures high data quality and fast turn-around for up to 48 samples per run (384-well format).

One-stop-shop: contains all necessary reagents and quality controls required to generate high-quality results. A software application allows instant transformation of raw data into easily interpretable figures and tables.

Broad applicability: analysis of miR-122-5p, miR-192-5p, and miR-151a-5p informs on liver function in the context of chronic or acute liver diseases: e.g. drug-induced liver injury (DILI), non-alcoholic fatty liver disease (NAFLD), and obesity.

assay requirements

Sample type: 200 μl fresh or frozen platelet-free plasma.

Required equipment: a RT-qPCR instrument (read the manual to find out which instruments are supported)

Not familiar with RT-qPCR?
Contact us to take advantage of our analytical services.

hepatomiR

product description

  • High throughput: Analysis of up to 12 samples per 96-well or 48 samples per 384-well plate.
  • Sample volume:
    • 200 µL platelet-poor plasma (lower volumes can be used for RUO purposes)
    • Samples can be deep-frozen (-70°C or lower) or fresh (centrifuge plasma within 2 hours of blood collection)
  • Efficiency by design: primer-coated 96- or 384-well plates reduce hands-on time and technical variability.
  • Fast and simple data analysis: the hepatomiR® software application simplifies data analysis. Going from raw data to final results takes <5 minutes.

Further information about the components included can be found here

The hepatomiR® software application is intended for:

  • Standardized analysis of qPCR data
  • Import: upload of raw data files from qPCR instrument and automatic Cq-value calling
  • Quality Control: inspect amplification curves, melting temperature, spike-in and hemolysis controls
  • Analysis: calculation and interpretation of hepatomiR® risk score
  • Export: export of raw Cq-values and hepatomiR®  risk score

The hepatomiR® software application and further information will be made available to all registered customers of the hepatomiR® kit.

intended use

  • The hepatomiR® test is intended for the quantitation of three human microRNAs in platelet-poor plasma. Using a proprietary algorithm, a risk score is computed that informs about the liver function of HCC, CCC, or mCRC patients and their risk of postoperative liver dysfunction.
  • Alternative (research-use) applications of the hepatomiR® test include the prognosis, diagnosis, staging, monitoring and treatment response of liver and metabolic diseases:
      • NAFLD and NASH
      • Cardiometabolic syndrome
      • Type-2 diabetes

Key Publications

Starlinger P, Hackl H, Pereyra D, Skalicky S, Geiger E, Finsterbusch M, Tamandl D, Brostjan C, Grünberger T, Hackl M, Assinger A. (2019). Predicting Postoperative Liver Dysfunction Based on Blood Derived MicroRNA Signatures. Hepatology. 2019 Jun;69(6):2636-2651. doi: 10.1002/hep.30572. Epub 2019 Apr 10

downloads

hepatomiR Manual

price list

product number product size PCR Cycler compatibility* price** product Information
KT-031-HT 96A RUO hepatomiR®
96-well		 48 samples
8 miRs/sample		 ABI, Bio-Rad, Eppendorf, Stratagene, Takara request quote  manual
KT-031-HT 96C RUO hepatomiR®
96-well		 48 samples
8 miRs/sample		 ABI (fast block)  request quote  manual
KT-031-HT 96D RUO hepatomiR®
96-well		 48 samples
8 miRs/sample		 Bio-Rad, Stratagene, DNA Engine  request quote  manual
KT-031-HT 96F RUO hepatomiR®
96-well		 48 samples
8 miRs/sample		 Roche LC480 I and II  request quote  manual
KT-031-HT 384E RUO hepatomiR®
384-well		 48 samples
8 miRs/sample		 ABI, Bio-Rad CFX 384™  request quote  manual
KT-031-HT 384G RUO hepatomiR®
384-well		 48 samples
8 miRs/sample		 Roche LC480 I and II  request quote  manual

* for detailled information on supported cyclers please click here
** Product launch Spring 2022

frequently asked questions (FAQ)

One kit provides sufficient reagents for the analysis of 48 samples.

The hepatomiR® kit is intended to be used with human samples. However, orthologs for all hepatomiRs exist in other species, enabling the use of the tests in non-mammalian species for research-use purposes. Importantly, the hepatomiR p-score has not been validated for non-human samples. To assess the feasibility of such projects, please contact us before using the assay in non-human species.

We have performed an in-depth analysis of the correlations of hepatomiRs to parameters such as liver enzymes and ICG clearance. Read our key publication to find out how hepatomiR performs in comparison to these parameters.

A wide range of qPCR cyclers equipped with 96well/384well-blocks are compatible with hepatomiR® plates. For detailled information on supported cyclers please click here.

5 control primers to monitor assay (RNA isolation, reverse transcription, and PCR efficiency) and sample quality (hemolysis) are included in the kit, no further controls are necessary.

Please contact us to discuss the possibilities.

All our kits include a protocol and reagents for RNA extraction. TAmiRNA´s hepatomiR® kit has been standardized to a volume of 200 µL plasma as starting material to ensure high RNA yield and prevent inhibition of downstream PCR amplification using the Serum/Plasma RNA extraction kit, which is included in the hepatomiR® kit. For further information read our guidelines detailing RNA purification and sample preparation for your hepatomiR® kit​.

LOD: 10 copies/µl (6 replicates, 95% positive detection); LLOQ: 35 copies/µl

Intra-assay (n=6) ≤ 20%, Inter-assay (n=3) ≤ 20%

We recommend using the software included with the kit for normalization and quality control.

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microRNA Biomarkers of Liver Function and Disease
microRNA Biomarkers of Liver Function and Disease