toxomiR
toxomiR_microRNA_biomarkers_of_toxicity

toxomiR®

microRNA biomarkers of toxicity

The term toxomiR® refers to a selection of 19 microRNAs that are enriched in various tissues. Toxicant-induced changes in the transcription and secretion of toxomiRs® are highly sensitive to toxic effects occuring in the liver, kidney, central nervous system, heart, muscle, lung, pancreas, vascular injury, and due to endocrine disruption. ToxomiRs® can be analyzed in serum or plasma.

This review recently published, gives detailed insight into the clinical utility of circulating microRNAs as biomarkers of toxicity.

toxomiR®
€ 147.– / sample

toxomiR®
€ 147.– / sample

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benefits

The toxomiR® panel is available as in-house service from TAmiRNA.

Non-invasive screening of toxic effects using serum or plasma.

MicroRNAs in human biofluids for early detection of tissue injury after exposure to toxic substances.

High sequence conservation makes cross-species application possible.

Rapid and robust solution for quantifying toxic effects in human plasma samples.

Customized service available with analysis targeted on specific set of microRNAs or tissue types.

service requirements

Frozen serum or plasma sample aliquots of at least 200 μl.

intended use

toxomiRs® can be used in pre-clinical and clinical phases of drug safety assessment. The microRNAs panel enable early and reliable detection as well as monitoring of adverse events.

The toxomiR® panel can be used:

  • to understand toxic mechanisms in liver, kidney, central nervous system, heart, muscle, lung, pancreas, vascular injury, and due to endocrine disruption
  • for identifying and monitoring safety risks in drug development
  • for early detection of tissue injury

service description

  • the toxomiR® panel is only available as in-house service
  • 200 µL serum and plasma are recommended (lower volumes can be used but require pre-testing)
  • deep-frozen (-70°C or lower) serum and plasma samples can be used.
  • 19 toxomiRs® and 5 quality controls are measured per sample
  • ready-to-use data for presentation and publication will be provided

dataset

the toxomiR® service include:

  • standardized analysis of qPCR data
  • quality control: inspect amplification curves, melting temperature, spike-in and hemolysis controls
  • ready-to-use data for presentation and publication will be provided
  • post project consultations to discuss next steps are included
  • customization is possible: upon request the analysis can be targeted to a specific set of microRNAs/tissue

downloads

service folder
key publications

price list

sample number price/sample
n<=48 € 275.–
n<=96 € 194.–
n<=154 € 175.–
n<=192 € 156.–
n>192 € 147.–

frequently asked questions (FAQ)

Upon request the analysis can be targeted to a specific set of microRNAs/tissue.

The toxomiR® panel is intended to be used with human samples. However, orthologs for toxomiR® do exist in other species, allowing the use of adapted version of the Panel in non-mammalian species. To assess the feasibility of such projects, please contact us.

5 control primers to monitor assay and sample quality are included in the panel.

TAmiRNA´s toxomiR® panel has been standardized to a volume of 200 µL plasma as starting material to ensure high RNA yield and prevent inhibition of downstream PCR amplification using the Serum/Plasma RNA extraction kit. For further information read our guidelines detailing RNA purification and sample preparation for the toxomiR® kit​.

LOD: 10 copies/µl (6 replicates, 95% positive detection); LLOQ: 35 copies/µl

Intra-assay (n=6) ≤ 20%, Inter-assay (n=3) ≤ 2

click here for our testing service