toxomiR
toxomiR_microRNA_biomarkers_of_toxicity

toxomiR®

microRNA biomarkers of toxicity

The term toxomiR® refers to a selection of 19 microRNAs that are enriched in various tissues. Toxicant-induced changes in the transcription and secretion of toxomiRs® are highly sensitive to toxic effects occuring in the liver, kidney, central nervous system, heart, muscle, lung, pancreas, vascular injury, and due to endocrine disruption. ToxomiRs® can be analyzed in serum or plasma.

This review recently published, gives detailed insight into the clinical utility of circulating microRNAs as biomarkers of toxicity.

toxomiR®
€ 147.– / sample

toxomiR®
€ 147.– / sample

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benefits

the toxomiR® panel are available as in-house service by TAmiRNA

non-invasive screening of toxic effects using serum or plasma

microRNAs in human biofluids for early detection of tissue injury after exposure to toxic substances.

Cross-species application is possible due the high sequence conservation.

rapid and robust solution to quantify toxic effects in human plasma samples

customization is possible: upon request the analysis can be targeted to a specific set of microRNAs/tissue

service requirements

Frozen serum or plasma sample aliquots of at least 200 μl.

intended use

toxomiRs® can be used in pre-clinical and clinical phases of drug safety assessment. The microRNAs panel enable early and reliable detection as well as monitoring of adverse events.

The toxomiR® panel can be used:

  • to understand toxic mechanisms in liver, kidney, central nervous system, heart, muscle, lung, pancreas, vascular injury, and due to endocrine disruption
  • for identifying and monitoring safety risks in drug development
  • for early detection of tissue injury

service description

  • the toxomiR® panel is only available as in-house service
  • 200 µL serum and plasma are recommended (lower volumes can be used but require pre-testing)
  • deep-frozen (-70°C or lower) serum and plasma samples can be used.
  • 19 toxomiRs® and 5 quality controls are measured per sample
  • ready-to-use data for presentation and publication will be provided

dataset

the toxomiR® service include:

  • standardized analysis of qPCR data
  • quality control: inspect amplification curves, melting temperature, spike-in and hemolysis controls
  • ready-to-use data for presentation and publication will be provided
  • post project consultations to discuss next steps are included
  • customization is possible: upon request the analysis can be targeted to a specific set of microRNAs/tissue

downloads

service folder
key publications

price list

sample number price/sample
n<=48 € 275.–
n<=96 € 194.–
n<=154 € 175.–
n<=192 € 156.–
n>192 € 147.–

frequently asked questions (FAQ)

Upon request the analysis can be targeted to a specific set of microRNAs/tissue.

The toxomiR® panel is intended to be used with human samples. However, orthologs for toxomiR® do exist in other species, allowing the use of adapted version of the Panel in non-mammalian species. To assess the feasibility of such projects, please contact us.

5 control primers to monitor assay and sample quality are included in the panel.

TAmiRNA´s toxomiR® panel has been standardized to a volume of 200 µL plasma as starting material to ensure high RNA yield and prevent inhibition of downstream PCR amplification using the Serum/Plasma RNA extraction kit. For further information read our guidelines detailing RNA purification and sample preparation for the toxomiR® kit​.

LOD: 10 copies/µl (6 replicates, 95% positive detection); LLOQ: 35 copies/µl

Intra-assay (n=6) ≤ 20%, Inter-assay (n=3) ≤ 2

click here for our testing service