microRNA biomarkers of platelet function
microRNA biomarkers of platelet function

thrombomiR®

microRNA biomarkers of platelet function

Circulating platelet microRNAs (thrombomiRs®) are novel biomarkers for intrinsic and on-treatment platelet reactivity. thrombomiRs® are secreted from platelets during their activation and can be detected in plasma and serum. Commonly used platelet function tests e.g. LTA, VerifyNow® or PFA-100®, require fresh blood and agonists. Therefore, they clearly lack standardization and their analytical variability may induce misinterpretations. The thrombomiR® kit enables an in vivo measure of platelet function, independently of the activation pathway.

microRNA biomarkers of platelet function
microRNA biomarkers of platelet function

thrombomiR® kit

Kit Price: price on request

microRNA biomarkers of platelet function
microRNA biomarkers of platelet function

thrombomiR kit

Kit Price: price on request

microRNA biomarkers of platelet function

benefits

A one-stop-shop solution to quickly start platelet microRNA biomarker research.

Each kit contains all quality controls required to perform high-quality microRNA biomarker analyses.

1 kit is sufficient for the analysis of 10 distinct platelet microRNAs, 3 endogenous control microRNAs, and 3 synthetic controls in 48 plasma samples.

Cross-species application is possible due to the high sequence conservation of platelet microRNAs.

Fast and simple data analysis using the thrombomiR analysis toolkit

assay requirements

Frozen platelet poor plasma (PPP) aliquots of at least 200 µl

qPCR instrument (read the manual to find out which instruments are supported)

microRNA biomarkers of platelet function

product description

  • High throughput: One kit is sufficient for the analysis of 48 platelet poor plasma (PPP) samples
  • Low sample volume: 200 µL PPP are recommended (lower volumes can be used but require pre-testing), (-70°C or lower) PPP samples can be used (stability for several years has been demonstrated)
  • Reduced hands-on time: primer coated 96 or 384 well plates
  • 10 thrombomiR®s and 6 controls/sample are measured per sample
  • Fast and simple data analysis: the web-based thrombomiR® software application enables easy data normalization and quality control

For further information about the components included can be found here

thrombomiR_mode_of_action

Software application

The thrombomiR® analysis toolkit enables

  • standardized analysis of qPCR data
  • import: upload of raw data files from qPCR instrument and automatic Cq-value calling
  • quality control: inspect amplification curves, melting temperature, spike-in and hemolysis controls
  • normalization: correction of technical variance and optional imputation of missing values
  • export: export normalized Cq-values of all samples and combine automatically with clinical parameters (i.e. age, gender, etc.)

The thrombomiR® analysis toolkit and further information will be made available to all registered customers of the thrombomiR® kit.

intended use

monitor the drug effects on platelet function, reactivity and hemostasis

diagnosis of platelet-related disorders

gain insights into the molecular function of platelets in vivo

determine the effect of a treatment on platelet reactivity in vivo

study the utility of platelet microRNAs as risk factors for cardiovascular and metabolic diseases

Low_levels_of_thrombomiRs_in_serum_are_associated_with_lower_risk_of_cardiovascular_death
correlation_between_ex_vivo_platelet_aggregation_tests_and_platelet_derived_microRNAs

Key Publications

Plasma levels of platelet-enriched microRNAs change during antiplatelet therapy in healthy subjects Krammer TL., Kollars M., Kyrle PA. et al. Front Pharmacol. 2022 Dec 12;13:1078722, doi: 10.3389/fphar.2022.1078722

Comprehensive Characterization of Platelet-Enriched MicroRNAs as Biomarkers of Platelet Activation Krammer TL, Zeibig S, Schrottmaier W et al. Cells 2022, 11(8), 1254 https://doi.org/10.3390/cells11081254 

Platelets and inflammation – insights from platelet non-coding RNA content and release in the Bruneck Study and the PACMAN-AMI trial Gutmann C, Barwari T, Schulte C et al. Cardiovascular Research, cvaf100, https://doi.org/10.1093/cvr/cvaf100

price list

product number product size PCR Cycler compatibility* price product Information
KT-021-TR 96A thrombomiR®
96-well		 48 samples
16 miRs/sample		 ABI, Bio-Rad, Eppendorf, Stratagene, Takara

price on request

 product sheet / manual
KT-021-TR 96C thrombomiR®
96-well		 48 samples
16 miRs/sample		 ABI (fast block) price on request product sheet / manual
KT-021-TR 96D thrombomiR®
96-well		 48 samples
16 miRs/sample		 Bio-Rad, Stratagene, DNA Engine price on request product sheet / manual
KT-21-TR 96F thrombomiR®
96-well		 48 samples
16 miRs/sample		 Roche LC480 I and II price on request product sheet / manual
KT-021-TR 384E thrombomiR®
384-well		 48 samples
16 miRs/sample		 ABI, Bio-Rad CFX 384™ price on request product sheet / manual
KT-021-TR 384G thrombomiR®
384-well		 48 samples
16 miRs/sample		 Roche LC480 I and II price on request product sheet / manual

* for detailed information on supported cyclers please click here

frequently asked questions (FAQ)

One kit provides sufficient reagents for the analysis of 48 serum or plasma samples. Upon request, we can provide smaller kits for proof-of-concept studies involving 10 samples or less.

The thrombomiR® kit is intended to be used with human samples. However, orthologs for thrombomiR®s do exist in other species, allowing the use of adapted version of the tests in non-mammalian species. To assess the feasibility of such projects, please contact us before using the assay in non-human species.

A wide range of qPCR cyclers equipped with 96well/384well-blocks are compatible with thrombomiR® plates. For detailled information on supported cyclers please click here.

5 control primers to monitor assay and sample quality are included in the kit, no further controls are necessary.

Please contact us to discuss the possibilities.

All our kits include a protocol and reagents for RNA extraction. TAmiRNA´s thrombomiR™ kit has been standardized to a volume of 200 µL plasma as starting material to ensure high RNA yield and prevent inhibition of downstream PCR amplification using the Serum/Plasma RNA extraction kit, which is included in the thrombomiR™ kit. For further information read our guidelines detailing RNA purification and sample preparation for your thrombomiR™ kit​.

LOD: 10 copies/µl (6 replicates, 95% positive detection); LLOQ: 35 copies/µl

Intra-assay (n=6) ≤ 20%, Inter-assay (n=3) ≤ 20%

We recommend using the software included in the kit for normalization and quality control.

microRNA biomarkers of platelet function
microRNA biomarkers of platelet function
microRNA biomarkers of platelet function