microRNA biomarkers of platelet function
microRNA biomarkers of platelet function

thrombomiR®

microRNA biomarkers of platelet function

Circulating platelet microRNAs (thrombomiRs®) are novel biomarkers for intrinsic and on-treatment platelet reactivity. thrombomiRs® are secreted from platelets during their activation and can be detected in plasma and serum. Commonly used platelet function tests e.g. LTA, VerifyNow® or PFA-100®, require fresh blood and agonists. Therefore, they clearly lack standardization and their analytical variability may induce misinterpretations. The thrombomiR® kit enables an in vivo measure of platelet function, independently of the activation pathway.

microRNA biomarkers of platelet function
microRNA biomarkers of platelet function

thrombomiR® kit
€ 4,900.–

microRNA biomarkers of platelet function
microRNA biomarkers of platelet function

thrombomiR kit
€ 4,900.–

microRNA biomarkers of platelet function

benefits

A one-stop-shop solution to quickly start platelet microRNA biomarker research.

Each kit contains all quality controls required to perform high-quality microRNA biomarker analyses.

1 kit is sufficient for the analysis of 10 distinct platelet microRNAs, 3 endogenous control microRNAs, and 3 synthetic controls in 48 plasma samples.

Cross-species application is possible due to the high sequence conservation of platelet microRNAs.

Fast and simple data analysis using the thrombomiR analysis toolkit

assay requirements

Frozen platelet poor plasma (PPP) aliquots of at least 200 µl

qPCR instrument (read the manual to find out which instruments are supported)

microRNA biomarkers of platelet function

price list

product number product size PCR Cycler compatibility* price product Information
KT-021-TR 96A thrombomiR®
96-well
48 samples
16 miRs/sample
ABI, Bio-Rad, Eppendorf, Stratagene, Takara € 4,900.– product sheet / manual
KT-021-TR 96C thrombomiR®
96-well
48 samples
16 miRs/sample
ABI (fast block) € 4,900.– product sheet / manual
KT-021-TR 96D thrombomiR®
96-well
48 samples
16 miRs/sample
Bio-Rad, Stratagene, DNA Engine € 4,900.– product sheet / manual
KT-21-TR 96F thrombomiR®
96-well
48 samples
16 miRs/sample
Roche LC480 I and II € 4,900.– product sheet / manual
KT-021-TR 384E thrombomiR®
384-well
48 samples
16 miRs/sample
ABI, Bio-Rad CFX 384™ € 4,700.– product sheet / manual
KT-021-TR 384G thrombomiR®
384-well
48 samples
16 miRs/sample
Roche LC480 I and II € 4,700.– product sheet / manual

* for detailed information on supported cyclers please click here

frequently asked questions (FAQ)

One kit provides sufficient reagents for the analysis of 48 serum or plasma samples. Upon request, we can provide smaller kits for proof-of-concept studies involving 10 samples or less.

The thrombomiR® kit is intended to be used with human samples. However, orthologs for thrombomiR®do exist in other species, allowing the use of adapted version of the tests in non-mammalian species. To assess the feasibility of such projects, please contact us before using the assay in non-human species.

A wide range of qPCR cyclers equipped with 96well/384well-blocks are compatible with thrombomiR® plates. For detailled information on supported cyclers please click here.

5 control primers to monitor assay and sample quality are included in the kit, no further controls are necessary.

All our kits include a protocol and reagents for RNA extraction. TAmiRNA´s thrombomiR™ kit has been standardized to a volume of 200 µL plasma as starting material to ensure high RNA yield and prevent inhibition of downstream PCR amplification using the Serum/Plasma RNA extraction kit, which is included in the thrombomiR™ kit. For further information read our guidelines detailing RNA purification and sample preparation for your thrombomiR™ kit​.

LOD: 10 copies/µl (6 replicates, 95% positive detection); LLOQ: 35 copies/µl

Intra-assay (n=6) ≤ 20%, Inter-assay (n=3) ≤ 20%

We recommend using the software included in the kit for normalization and quality control.

microRNA biomarkers of platelet function
microRNA biomarkers of platelet function
microRNA biomarkers of platelet function