microRNA Biomarkers of Bone Quality
microRNA Biomarkers of Bone Quality

osteomiRTM

microRNA biomarkers of bone quality

The osteomiR® test enables parallel measurement of 19 carefully selected and validated microRNA biomarkers of bone quality in human serum samples. The osteomiR® software can be used for individual analysis of all 19 microRNA biomarkers, as well as for the calculation of a fracture-risk score. This score is based on multivariate microRNA model.

microRNA Biomarkers of Bone Quality
microRNA Biomarkers of Bone Quality

osteomiR® kit
€ 6,100.–

microRNA Biomarkers of Bone Quality
TAmiRNA_osteomiR_Box

osteomiR® kit
€ 6,100.-

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benefits

A one-stop-shop solution to start your microRNA bone and muscle biomarker research project.

Each kit contains all quality controls required to perform high-quality microRNA bone and muscle biomarker research.

A single kit enables the analysis of 19 osteomiR® microRNA bone biomarkers and 5 quality controls in 48 serum or plasma samples.

The high conservation of osteomiR sequences allows cross-species applications.

assay requirements

Frozen serum sample aliquots of at least 200 μl

qPCR instrument (read the manual to find out which instruments are supported)

microRNA Biomarkers of Bone Quality

price list

product number product size PCR Cycler compatibility* price product Information
KT-011-OT 96A osteomiR®
96-well
48 samples
24 miRs/sample
ABI, Bio-Rad, Eppendorf, Stratagene, Takara € 6,100.– product sheet / manual
KT-011-OT 96C osteomiR®
96-well
48 samples
24 miRs/sample
ABI (fast block) € 6,100.– product sheet / manual
KT-011-OT 96D osteomiR®
96-well
48 samples
24 miRs/sample
Bio-Rad, Stratagene, DNA Engine € 6,100.– product sheet / manual
KT-011-OT 96F osteomiR®
96-well
48 samples
24 miRs/sample
Roche LC480 I and II € 6,100.– product sheet / manual
KT-011-OT 384E osteomiR®
384-well
48 samples
24 miRs/sample
ABI, Bio-Rad CFX 384™ € 5,800.– product sheet / manual
KT-011-OT 384G osteomiR®
384-well
48 samples
24 miRs/sample
Roche LC480 I and II € 5,800.– product sheet / manual

* for detailled information on supported cyclers please click here

frequently asked questions (FAQ)

One kit provides sufficient reagents for the analysis of 48 serum samples.

The osteomiR® kit is intended to be used with human samples. However, orthologs for osteomiRs do exist in other species, allowing the use of adapted version of the tests in non-mammalian species. To assess the feasibility of such projects, please contact us before using the assay in non-human species.

We have performed an in-depth analysis of the correlations of osteomiRs to parameters of dynamic histomorphometry and nanoCT. The results from this analysis can be read here.

A wide range of qPCR cyclers equipped with 96well/384well-blocks are compatible with osteomiR® plates. For detailled information on supported cyclers please click here.

5 control primers to monitor assay and sample quality are included in the kit, no further controls are necessary.

All our kits include a protocol and reagents for RNA extraction. TAmiRNA´s osteomiR® kit has been standardized to a volume of 200 µL plasma as starting material to ensure high RNA yield and prevent inhibition of downstream PCR amplification using the Serum/Plasma RNA extraction kit, which is included in the osteomiR® kit. For further information read our guidelines detailing RNA purification and sample preparation for your osteomiR® kit​.

LOD: 10 copies/µl (6 replicates, 95% positive detection); LLOQ: 35 copies/µl

Intra-assay (n=6) ≤ 20%, Inter-assay (n=3) ≤ 20%

We recommend using the software included in the kit for normalization and quality control.

microRNA Biomarkers of Bone Quality
microRNA Biomarkers of Bone Quality
microRNA Biomarkers of Bone Quality