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osteomiRTM

microRNA Biomarkers of Bone Quality

The osteomiR™ test enables parallel measurement of 19 carefully selected and validated microRNA biomarkers of bone quality in human serum samples. The osteomiR™ software can be used for individual analysis of all 19 microRNA biomarkers, as well as for the calculation of a fracture-risk score. This score is based on multivariate microRNA model.

osteomiR test kit
osteomiR

osteomiR™ kit
€ 6,790.–
€ 8,390.–

temporary -20% product launch discount

oder_now
TAmiRNA_osteomiR_Box

osteomiR kit
€ 6,790.–
€ 8,390.–

temporary -20% product launch discount

oder_now

benefits

One-stop-shop solution to start microRNA bone and muscle research

Contains all quality controls required to perform high quality microRNA bone biomarker research

A single kit allows analysis of 19 osteomiR™ microRNA bone quality biomarkers in up to 48 samples.

Cross-species application is possible due the high sequence conservation

Assess bone quality using the osteomiR™ score.

assay requirements

Frozen serum sample aliquots of at least 200 μl.

qPCR instrument (read the manual to find out which instruments are supported).

testing service

product description

  • High throughput: Analysis of up to 48 serum samples
  • Low sample volume: 200 µL human serum (lower volumes can be used but require pre-testing), deep-frozen (-70°C or lower) serum samples can be used (stability for several years has been demonstrated)
  • Reduced hands-on time and technical varibility: primer coated 96 or 384 well plates
  • 19 osteomiRs and 5 quality controls are measured per sample
  • Fast and simple data analysis: the web-based osteomiR™ software application enables easy data normalization and quality control.

For further information about the components included can be found here .

The osteomiR™ analysis toolkit enables:

  • Standardized analysis of qPCR data
  • Import: upload of raw data files from qPCR instrument and automatic Cq-value calling
  • Quality Control: inspect amplification curves, melting temperature, spike-in and hemolysis controls
  • Normalization: correction of technical variance and optional imputation of missing values
  • Export: export normalized Cq-values of all samples and combine automatically with clinical parameters (i.e. age, gender, etc.)

The osteomiR™ analysis toolkit and further information will be made available to all registered customers of the osteomiR™ kit.

intended use

The osteomiR™ panel was designed based on a series of research studies, which provide evidence that osteomiRs

  • are associated to bone microstructure and histomorphometry,
  • are regulated in patients with osteoporotic fractures
  • are BMD-independent risk factors for osteoporotic fractures
  • regulate bone formation and resorption via multiple pathways
  • are novel biomarkers for bone diseases

Key Publications

The key publications leading to the identification of these novel microRNA biomarkers of bone quality can be found here

Key Publications

downloads

osteomiR folder
osteomiR manual
key publications
qPCR cycler compatibility
tech note 1 / osteomiR
tech note 2 / osteomiR

price list

product number product size PCR Cycler compatibility* price** product Information
KT-011-OT 96A osteomiR™
96-well
48 samples
24 miRs/sample
ABI, Bio-Rad, Eppendorf, Stratagene, Takara € 6,790.– product sheet / manual
KT-011-OT 96C osteomiR™
96-well
48 samples
24 miRs/sample
ABI (fast block) € 6,790.– product sheet / manual
KT-011-OT 96D osteomiR™
96-well
48 samples
24 miRs/sample
Bio-Rad, Stratagene, DNA Engine € 6,790.– product sheet / manual
KT-011-OT 96F osteomiR™
96-well
48 samples
24 miRs/sample
Roche LC480 I and II € 6,790.– product sheet / manual
KT-011-OT 384E osteomiR™
384-well
48 samples
24 miRs/sample
ABI, Bio-Rad CFX 384™ € 6,390.– product sheet / manual
KT-011-OT 384G osteomiR™
384-well
48 samples
24 miRs/sample
Roche LC480 I and II € 6,390.– product sheet / manual

* for detailled information on supported cyclers please click here
** temporary -20% product launch discount

frequently asked questions (FAQ)

One kit provides sufficient reagents for the analysis of 48 serum samples.

The osteomiR™ kit is intended to be used with human samples. However, orthologs for osteomiRs do exist in other species, allowing the use of adapted version of the tests in non-mammalian species. To assess the feasibility of such projects, please contact us before using the assay in non-human species.

We have performed an in-depth analysis of the correlations of osteomiRs to parameters of dynamic histomorphometry and nanoCT. The results from this analysis can be read here.

A wide range of qPCR cyclers equipped with 96well/384well-blocks are compatible with osteomiR plates. For detailled information on supported cyclers please click here.

5 control primers to monitor assay and sample quality are included in the kit, no further controls are necessary.

All our kits include a protocol and reagents for RNA extraction. TAmiRNA´s osteomiR™ kit has been standardized to a volume of 200 µL plasma as starting material to ensure high RNA yield and prevent inhibition of downstream PCR amplification using the Serum/Plasma RNA extraction kit, which is included in the osteomiR™ kit. For further information read our guidelines detailing RNA purification and sample preparation for your osteomiR™ kit​.

LOD: 10 copies/µl (6 replicates, 95% positive detection); LLOQ: 35 copies/µl

Intra-assay (n=6) ≤ 20%, Inter-assay (n=3) ≤ 20%

We recommend using the software included in the kit for normalization and quality control.

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