osteomiRTM
microRNA biomarkers of bone quality
The osteomiR® test enables parallel measurement of 19 carefully selected and validated microRNA biomarkers of bone quality in human serum samples. The osteomiR® software can be used for individual analysis of all 19 microRNA biomarkers, as well as for the calculation of a fracture-risk score. This score is based on multivariate microRNA model.
benefits
A one-stop-shop solution to start your microRNA bone and muscle biomarker research project.
Each kit contains all quality controls required to perform high-quality microRNA bone and muscle biomarker research.
A single kit enables the analysis of 19 osteomiR® microRNA bone biomarkers and 5 quality controls in 48 serum or plasma samples.
The high conservation of osteomiR sequences allows cross-species applications.
assay requirements
Frozen serum sample aliquots of at least 200 μl
qPCR instrument (read the manual to find out which instruments are supported)
product description
- High throughput: Analysis of up to 48 serum samples
- Low sample volume: 200 µL human serum (lower volumes can be used but require pre-testing), deep-frozen (-70°C or lower) serum samples can be used (stability for several years has been demonstrated)
- Reduced hands-on time and technical varibility: primer coated 96 or 384 well plates
- 19 osteomiRs and 5 quality controls are measured per sample
- Fast and simple data analysis: the web-based osteomiR® software application enables easy data normalization and quality control
For further information about the components included can be found here
The osteomiR® analysis toolkit enables:
- Standardized analysis of qPCR data
- Import: upload of raw data files from qPCR instrument and automatic Cq-value calling
- Quality Control: inspect amplification curves, melting temperature, spike-in and hemolysis controls
- Normalization: correction of technical variance and optional imputation of missing values
- Export: export normalized Cq-values of all samples and combine automatically with clinical parameters (i.e. age, gender, etc.)
The osteomiR® analysis toolkit and further information will be made available to all registered customers of the osteomiR® kit.
intended use
The osteomiR® panel was designed based on a series of research studies, which provide evidence that osteomiRs
- are associated to bone microstructure and histomorphometry
- are regulated in patients with osteoporotic fractures
- are BMD-independent risk factors for osteoporotic fractures
- regulate bone formation and resorption via multiple pathways
- are novel biomarkers for bone diseases
price list
| product number | product | size | PCR Cycler compatibility* | price | product Information |
|---|---|---|---|---|---|
| KT-011-OT 96A | osteomiR® 96-well |
48 samples 24 miRs/sample |
ABI, Bio-Rad, Eppendorf, Stratagene, Takara | € 6,100.– | product sheet / manual |
| KT-011-OT 96C | osteomiR® 96-well |
48 samples 24 miRs/sample |
ABI (fast block) | € 6,100.– | product sheet / manual |
| KT-011-OT 96D | osteomiR® 96-well |
48 samples 24 miRs/sample |
Bio-Rad, Stratagene, DNA Engine | € 6,100.– | product sheet / manual |
| KT-011-OT 96F | osteomiR® 96-well |
48 samples 24 miRs/sample |
Roche LC480 I and II | € 6,100.– | product sheet / manual |
| KT-011-OT 384E | osteomiR® 384-well |
48 samples 24 miRs/sample |
ABI, Bio-Rad CFX 384™ | € 5,800.– | product sheet / manual |
| KT-011-OT 384G | osteomiR® 384-well |
48 samples 24 miRs/sample |
Roche LC480 I and II | € 5,800.– | product sheet / manual |
* for detailled information on supported cyclers please click here
