Reverse-transcription quantitative PCR (RT-qPCR) is ideal for a sensitive and specific gene expression analysis including microRNAs, other small and large non-coding RNAs, as well as messenger RNA.
TAmiRNA is an expert in developing and applying RT-qPCR assays for various RNA types. Our microRNA platform takes advantage of target-specific LNA-enhanced (locked nucleic acid) forward and reverse primers with unmet specificity and sensitivity. We use this assay for parallel quantification of 1 up to 768 microRNAs per cDNA sample. The high sensitivity allows us to analyze low RNA input samples such as biofluids, cell culture supernatants, extracellular vesicles (EVs), and microdissected tissues without pre-amplification.