RT-qPCR services

RT-qPCR services

Reverse-transcription quantitative PCR (RT-qPCR) is ideal for a sensitive and specific gene expression analysis including microRNAs, other small and large non-coding RNAs, as well as messenger RNA. TAmiRNA is an expert in developing and applying RT-qPCR assays for various RNA types. Our microRNA platform takes advantage of target-specific LNA-enhanced (locked nucleic acid) forward and reverse primers with unmet specificity and sensitivity. We can use this assay for parallel quantification of 1 up to 768 microRNAs per cDNA sample. The high sensitivity allows us to analyze low RNA input samples such as biofluids, cell culture supernatants, or microdissected tissues without pre-amplification.

RT-qPCR services
RT-qPCR services

benefits

Our service includes RNA extraction, RT-qPCR, data analysis, and reporting of ready-to-publish results.

Our RT-qPCR services are available for microRNAs, other small and large non-coding RNAs as well as messenger RNAs.

Our RT-qPCR services can be applied during basic, translational, and pre-clinical research studies as well as clinical trials.

RT-qPCR analysis can be performed on cells, tissues, and biofluids from a long list of organisms, including:

  • Human
  • Non-human primates
  • Mouse
  • Rat
  • Pig
  • Cow
  • Horse
  • Dog
  • Chinese hamster

Our ability to develop customer-tailored assays, to perform analytical validation, and transfer validated assays to our customers complements our RT-qPCR services.

sample requirements

  • Biofluids: serum, plasma, urine, CSF, synovial fluid, milk, or cell culture supernatant.
  • Extracellular vesicles (EVs): we can isolate EVs in-house or work with material provided by our customers.
  • Cells and tissues: how low can we go? very low – 10,000 µm2 dissected tissue area or ~ 100 cells
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service description

  • Total RNA extraction from various biological matrices.
  • RT-qPCR analysis of microRNAs, small and large non-coding RNAs, and messenger RNAs.
  • Data analysis, reporting, and interpretation support.
  • Customer-tailored assay development.
  • Analytical validation.
  • Assay Transfer.

reference projects

MicroRNA profiling reveals distinct signatures in degenerative rotator cuff pathologies.

Plachel F, Heuberer P, Gehwolf R et al. J Orthop Res. 2019 Sep 14. doi: 10.1002/jor.24473

MicroRNAs in porcine uterus and serum are affected by zearalenone and represent a new target for mycotoxin biomarker discovery.

Grenier B, Hackl M, Skalicky S et al. Sci Rep. 2019 Jun 28;9(1):9408. doi: 10.1038/s41598-019-45784-x

Biotransformation of the Mycotoxin Zearalenone to its Metabolites Hydrolyzed Zearalenone (HZEN) and Decarboxylated Hydrolyzed Zearalenone (DHZEN) Diminishes its Estrogenicity In Vitro and In Vivo.

Fruhauf S, Novak B, Nagl V et al. Toxins (Basel). 2019 Aug 20;11(8). pii: E481. doi: 10.3390/toxins11080481

SVF-derived extracellular vesicles carry characteristic miRNAs in lipedema.

Priglinger E, Strohmeier K, Weigl M et al. Sci Rep. 2020 Apr 29;10(1):7211. doi: 10.1038/s41598-020-64215-

Unique, Gender-Dependent Serum microRNA Profile in PLS3 Gene-Related Osteoporosis

Mäkitie RE., Hackl M., Weigl M et al. J Bone Miner Res. 2020 May 26. doi: 10.1002/jbmr.4097.

MicroRNA Expression Profile Changes after Cardiopulmonary Bypass and Ischemia/Reperfusion-Injury in a Porcine Model of Cardioplegic Arrest

Kiss A., Heber S., Kramer A-M et al. Diagnostics 2020, 10, 240; doi:10.3390/diagnostics10040240.

MicroRNA levels in bone and blood change during bisphosphonate and teriparatide therapy in an animal model of postmenopausal osteoporosis

Kocijan R, Weigl M, Skalicky S et al. Bone 2019 Nov 1:115104. doi: 10.1016/j.bone.2019.115104.

Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues

Skalicky S, Zwiers PJ, Kuiper T et al. Biotechniques. 2019 Oct 17. doi: 10.2144/btn-2019-0032. Epub 2019 Oct 17

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RT-qPCR services
RT-qPCR services