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Aged-vascular niche hinders osteogenesis of mesenchymal stem cells through paracrine repression of Wnt-axis
Fleischhacker V, Milosic F, Bricelj M et al. Aging Cell 2024,

Sepsis induces heterogeneous transcription of coagulation- and inflammation-associated genes in renal microvasculature
Luxen M, Zwiers PJ, Jongmann RM et al. Thrombosis Research. March 18, 2024DOI:

Analysis of extracellular vesicle microRNA profiles reveals distinct blood and lymphatic endothelial cell origins
Pultar M, Oesterreicher J, Hartmann J et al. J of Extracellular Bio.2024;3:e134;

MicroRNA-30d-5p—A Potential New Therapeutic Target for Prevention of Ischemic Cardiomyopathy after Myocardial Infarction
Boxhammer E, Paar V, Wernly B et al. Cells 2023, 12(19), 2369;

Circulating microRNAs in young individuals with long-duration type 1 diabetes in comparison with healthy controls. Swolin-Eide D, Forsander G, Pundziute Lyckå A et al. Sci Rep. 2023 Jul 19;13(1):11634. doi: 10.1038/s41598-023-38615-7.

Comprehensive mRNA-sequencing-based characterization of three HEK-293 cell lines during an rAAV production process for Gene Therapy Applications
Pistek M., Kahlig CI., Hackl M. et al. Biotechnol J. 2023 May 16;e2200513. doi: 10.1002/biot.202200513 

Plasma levels of platelet-enriched microRNAs change during antiplatelet therapy in healthy subjects

Krammer TL., Kollars M., Kyrle PA. et al. Front Pharmacol. 2022 Dec 12;13:1078722, doi: 10.3389/fphar.2022.1078722

Small non-coding RNA landscape of extracellular vesicles from a post-traumatic model of equine osteoarthritis

Anderson JR., Jacobsen S., Walters M. et al. Front. Vet. Sci., 08 August 2022, Sec. Veterinary Regenerative Medicine,

Comprehensive Characterization of Platelet-Enriched MicroRNAs as Biomarkers of Platelet Activation

Krammer TL, Zeibig S, Schrottmaier W et al. Cells 2022, 11(8), 1254 

miND (miRNA NGS Discovery pipeline): a small RNA-seq analysis pipeline and report generator for microRNA biomarker discovery studies

Diendorfer A, Khamina K, Pultar M and Hackl M. F1000Research 2022, 11:233, 1226; 

A MicroRNA Next-Generation-Sequencing Discovery Assay (miND) for Genome-Scale Analysis and Absolute Quantitation of Circulating MicroRNA Biomarkers

Khamina K, Diendorfer A, Skalicky S, et al. Int. J. Mol. Sci. 2022, 23(3), 1226; 

Association of cardiometabolic microRNAs with COVID-19 severity and mortality

Gutmann C, Khamina K, Theofilatos K, et al. Cardiovasc Res. 2022 Feb; 118(2): 461–474.. doi: 10.1093/cvr/cvab338

Systems analysis of miRNA biomarkers to inform drug safety

Schofield AL, Brown JP, Brown J, et al. Arch Toxicol. 2021 Nov;95(11):3475-3495. doi: 10.1007/s00204-021-03150-9

Longitudinal Changes of Circulating miRNAs during Bisphosphonate and Teriparatide Treatment in an Animal Model of Postmenopausal Osteoporosis

Weigl M, Kocijan R, Ferguson J, et al. JBMR. 2021 Feb 17. doi: 10.1002/jbmr.4276. Online ahead of print.

Circulating miRNAs in bone health and disease

Grillari J, Mäkitie RE, Kocijan R, et al. Bone. Volume 145, April 2021, 115787.

Reiterative infusions of MSCs improve pediatric osteogenesis imperfecta eliciting a pro-osteogenic paracrine response: TERCELOI clinical trail

Infante A., Gener B., Vázquez M et al. Clin Transl Med 2021 Jan;11(1):e265. doi: 10.1002/ctm2.265.

Diagnostic Performance of a Panel of miRNAs (OsteomiR) for Osteoporosis in a Cohort of Postmenopausal Women

Kerschan-Schindl K., Hackl M., Boschitsch E et al.  Calcified Tissue International. 2021-01-11. doi:10.1007/s00223-020-00802-3.

microRNA-146a controls age-related bone loss.

Saferding V., Hofmann M., Brunner JS et al.  Aging Cell. 2020 Nov;19(11):e13244. doi: 10.1111/acel.13244.

miRNA-21 deficiency impairs alveolar socket healing in mice

Strauss FJ., Stähli A., Kobatake R et al.  J Periodontol Rep. 2020 May 12. doi: 10.1002/JPER.19-0567

Unique, Gender-Dependent Serum microRNA Profile in PLS3 Gene-Related Osteoporosis

Mäkitie RE., Hackl M., Weigl M et al. J Bone Miner Res. 2020 May 26. doi: 10.1002/jbmr.4097.

SVF-derived Extracellular Vesicles Carry Characteristic miRNAs in Lipedema

Priglinger E., Strohmeier K., Weigl M et al.  Sci Rep. 2020 Apr 29;10(1):7211. doi: 10.1038/s41598-020-64215-

MicroRNAs as Promising Biomarkers of Platelet Activity in Antiplatelet Therapy Monitoring

Krammer TL., Mayr M & Hackl M. Int. J. Mol. Sci. 2020, 21(10), 3477;

MicroRNA Expression Profile Changes after Cardiopulmonary Bypass and Ischemia/Reperfusion-Injury in a Porcine Model of Cardioplegic Arrest

Kiss A., Heber S., Kramer A-M et al. Diagnostics 2020, 10, 240; doi:10.3390/diagnostics10040240.

Evaluation of a Panel of MicroRNAs that Predicts Fragility Fracture Risk: A Pilot Study

Ladang A., Beaudart C., Locquetet al. Calcif Tissue Int. 2019 Nov 15. doi: 10.1007/s00223-019-00628-8.

Roux-en-Y-Bariatric Surgery Reduces Markers of Metabolic Syndrome in Morbidly Obese Patients

Rega-Kaun G., Kaun C., Jaegersberger G et al. Obes Surg 2019 Nov 15. doi: 10.1007/s11695-019-04190-y.

MicroRNA levels in bone and blood change during bisphosphonate and teriparatide therapy in an animal model of postmenopausal osteoporosis

Kocijan R, Weigl M, Skalicky S et al. Bone 2019 Nov 1:115104. doi: 10.1016/j.bone.2019.115104.

Serum microRNA as novel biomarkers for osteoporotic vertebral fractures

Zarecki P, Hackl M, Grillari j, et al. Bone 2019 Oct 24:115105. doi: 10.1016/j.bone.2019.115105.  

Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues

Skalicky S, Zwiers PJ, Kuiper T et al. Biotechniques. 2019 Oct 17. doi: 10.2144/btn-2019-0032. [Epub ahead of print]

MicroRNA profiling reveals distinct signatures in degenerative rotator cuff pathologies.

Plachel F, Heuberer P, Gehwolf R et al. J Orthop Res. 2019 Sep 14. doi: 10.1002/jor.24473

Biotransformation of the Mycotoxin Zearalenone to its Metabolites Hydrolyzed Zearalenone (HZEN) and Decarboxylated Hydrolyzed Zearalenone (DHZEN) Diminishes its Estrogenicity In Vitro and In Vivo.

Fruhauf S, Novak B, Nagl V et al. Toxins (Basel). 2019 Aug 20;11(8). pii: E481. doi: 10.3390/toxins11080481

Differential MicroRNA expression following head-down tilt bed rest: implications for cardiovascular responses to microgravity.

Ade CJ and Bemben DA Physiol Rep. 2019 May;7(9):e14061. doi: 10.14814/phy2.14061

MicroRNAs in porcine uterus and serum are affected by zearalenone and represent a new target for mycotoxin biomarker discovery.

Grenier B, Hackl M, Skalicky S et al. Sci Rep. 2019 Jun 28;9(1):9408. doi: 10.1038/s41598-019-45784-x.

Predicting Postoperative Liver Dysfunction Based on Blood Derived MicroRNA Signatures.

Starlinger P, Hackl H, Pereyra D et al. Hepatology. 2019 Jun;69(6):2636-2651. doi: 10.1002/hep.30572. Epub 2019 Apr 10.

Catalyzing Transcriptomics Research in Cardiovascular Disease: The CardioRNA COST Action CA17129

Pedrosa da C. Gomes C, Ágg B, Andova A et al Non-Coding RNA 2019, 5(2), 31

St Thomas’ Hospital Polarizing blood cardioplegia improves hemodynamic recovery in a porcine model of cardiopulmonary Bypass.

Santer D, Kramer A, Kiss A et al. The Journal of Thoracic and Cardiovascular Surgery, available online 12 December 2018

Reduction of Premature Aging Markers After Gastric Bypass Surgery in Morbidly Obese Patients.

Hohensinner PJ, Kaun C, Ebenbauer B, Hackl M et al. Obes Surg. 2018 Apr 25. doi: 10.1007/s11695-018-3247-3.

Bone-related circulating microRNAs miR-29b-3p, miR-550a-3p and miR-324-3p and their Association to Bone Microstructure and Histomorphometry. 

Feichtinger X, Muschitz C, Heimel P, Baierl A, Fahrleitner-Pammer A, Redl H et al. Scientific Reports, Vol 8 , Article number: 4867 (2018)

Altered MicroRNA Profile in Osteoporosis Caused by Impaired WNT Signaling 

Mäkitie RE, Hackl M, Niinimäki R,Kakko S, Grillari J, Mäkitie O J Clin Endocrinol Metab. 2018 Mar 1. doi: 10.1210/jc.2017-02585.

Cost-utility analysis of fracture risk assessment using microRNA compared with Standard Tools and no Monitoring in the Austrian female Population

Walter E, Dellago H, Grillari J, Dimai HP, Hackl M Bone. Volume 108, 2018; Pages 44-54.

MicroRNAs and toxicology: A love marriage

Schraml E, Hackl M and Grillari J. Toxicology Reports. Volume 4, 2017; Pages 634-636.

Clopidogrel in critically ill patients.

Schoergenhofer C, Hobl EL, Schellongowski P, Heinz G, Speidl W, et al Clin Pharmacol Ther. 2017 Sep 15.

Serum microRNAs Are Indicative of Skeletal Fractures in Postmenopausal Women with and without Type 2 Diabetes and Influence Osteogenic and Adipogenic Differentiation of Adipose-Tissue Derived Mesenchymal Stem Cells In Vitro.

Heilmeier U, Hackl M, Skalicky S, Weilner S, Schroeder F, Vierlinger K, et al. J Bone Miner Res 2016. Dec;31(12):2173-2192

Circulating microRNA signatures in patients with idiopathic and postmenopausal osteoporosis and fragility fractures.

Kocijan R, Muschitz C, Geiger E, Skalicky S, Baierl A, Dormann R, et al. J Clin Endocrinol Metab 2016:jc.2016-2365.

Vesicular Galectin-3 levels decrease with donor age and contribute to the reduced osteo-inductive potential of human plasma derived extracellular vesicles.

Weilner S, Keider V, Winter M, Harreither E, Salzer B, Weiss F, Schraml E, et al. Aging (Albany NY). 2016 Jan;8(1):16-33.

Urine is a novel source of autologous mesenchymal stem cells for patients with epidermolysis bullosa.

Schosserer M, Reynoso R, Wally V, Jug B, Kantner V, Weilner S, et al. BMC Res Notes. 2015 Dec 10;8(1):767.

Circulating microRNAs as novel biomarkers for bone diseases – Complex signatures for multifactorial diseases?

Hackl M, Heilmeier U, Weilner S, Grillari J. Mol Cell Endocrinol. 2016 Sep 5;432:83-95.

Differentially circulating miRNAs after recent osteoporotic fractures can influence osteogenic Differentiation.

Weilner S, Skalicky S, Salzer B, Keider V, Wagner M, Hildner F, et al. Bone. 2015 May 28;79:43-51.

Annotation of additional evolutionary conserved microRNAs in CHO cells from updated genomic data.

Diendorfer AB, Hackl M, Klanert G, Jadhav V, Reithofer M, Stiefel F, et al. Biotechnol Bioeng. 2015 Jul;112(7):1488-93.

Molecular and cellular effects of in vitro shockwave treatment on lymphatic endothelial cells. 

Rohringer S, Holnthoner W, Hackl M, Weihs AM, Rünzler D, Skalicky S, et al. PLoS One. 2014 Dec 11;9(12):e114806.

MicroRNAs differentially present in the plasma of HIV elite controllers reduce HIV infection in vitro.

Reynoso R, Laufer N, Hackl M, Skalicky S, Monteforte R, Turk G, Carobene M, et al. Scientific Reports, 2014 Aug 1;4:5915.

Identification of microRNAs specific for high producer CHO cell lines using steady-state cultivation.

Maccani A, Hackl M, Leitner C, Steinfellner W, Graf AB, Tatto NE, et al. Appl Microbiol Biotechnol. 2014 Sep;98(17):7535-48.

Stable overexpression of miR-17 enhances recombinant protein production of CHO cells.

Jadhav V, Hackl M, Klanert G, Hernandez Bort JA, Kunert R, Grillari J, Borth N. J Biotechnol. 2014 Apr 10;175:38-44.

Analysis of microRNA transcription and post-transcriptional processing by Dicer in the context of CHO cell Proliferation.

Hackl M, Jadhav V, Klanert G, Karbiener M, Scheideler M, Grillari J, Borth N. J Biotechnol. 2014 Nov 20;190:76-84.

Endogenous microRNA clusters outperform chimeric sequence clusters in Chinese hamster ovary cells.n.

Klanert G, Jadhav V, Chanoumidou K, Grillari J, Borth N, Hackl M. Biotechnol J. 2014 Apr;9(4):538-44.

2013 and earlier

Review of the importance of microRNAs in mammalian cell culture technology

Jadhav V, Hackl M, Druz A, Shridhar S, Chung CY, Heffner KM, Kreil DP, Betenbaugh M, Shiloach J, Barron N, Grillari J, Borth N. CHO microRNA engineering is growing up: recent successes and future challenges (2013). Biotechnol Adv. 31(8):1501-13. Review.  

PubMed PMID: 23916872

Review of the importance of secreted microRNAs during aging of cells, tissues and entire organisms.

Weilner, S., Schraml, E., Redl, H., Grillari-Voglauer, R., Grillari, J. Secretion of microvesicular miRNAs in cellular and organismal aging (2013) Experimental Gerontology, 48 (7), pp. 626-633.

PubMed PMID:23283304

Original research article on the role of miR-21 in cellular lifespan

Dellago, H., Preschitz-Kammerhofer, B., Terlecki-Zaniewicz, L., Schreiner, C., Fortschegger, K., Chang, M.W.-F., Hackl, M., Monteforte, R., Kühnel, H., Schosserer, M., Gruber, F., Tschachler, E., Scheideler, M., Grillari-Voglauer, R., Grillari, J., Wieser, M. High levels of oncomiR-21 contribute to the senescence-induced growth arrest in normal human cells and its knock-down increases the replicative lifespan (2013) Aging Cell, 12 (3), pp. 446-458.

PubMed PMID: 23496142

Bioinformatic prediction of piRNAs – a new class of small non-coding RNAs – in a common recombinant therapeutic production cell line

Gerstl, M.P., Hackl, M., Graf, A.B., Borth, N., Grillari, J. Prediction of transcribed PIWI-interacting RNAs from CHO RNAseq data (2013) Journal of Biotechnology, 166 (1-2), pp. 51-57.

PubMed PMID: 23639388

Original research article on the relevance of microRNAs in mediating the effect of UVB-irradiation on fibroblasts.

Greussing, R., Hackl, M., Charoentong, P., Pauck, A., Monteforte, R., Cavinato, M., Hofer, E., Scheideler, M., Neuhaus, M., Micutkova, L., Mueck, C., Trajanoski, Z., Grillari, J., Jansen-Dürr, P. Identification of microRNA-mRNA functional interactions in UVB-induced senescence of human diploid fibroblasts (2013) BMC Genomics, 2013 Apr 4;14:224.

PubMed PMID: 23557329

Protocol describing the isolation and subsequent reprogramming of urine cell into pluripotent stem cells.

Zhou, T., Benda, C., Dunzinger, S., Huang, Y., Ho, J.C., Yang, J., Wang, Y., Zhang, Y., Zhuang, Q., Li, Y., Bao, X., Tse, H.-F., Grillari, J., Grillari-Voglauer, R., Pei, D., Esteban, M.A. Generation of human induced pluripotent stem cells from urine samples (2012) Nature Protocols, 7 (12), pp. 2080-2089.

PubMed PMID: 23138349

Opinion Article on the use of microRNAs in engineering cell lines to become better tools for the production of recombinant therapeutic proteins

Hackl, M., Borth, N., Grillari, J. MiRNAs – pathway engineering of CHO cell factories that avoids translational burdening (2012) Trends in Biotechnology, 30 (8), pp. 405-406.

PubMed PMID:22673691

Orignial research article on a microRNA that controls DNA Damage in T-Cells in response to chemotherapeutic treatment with Etoposide

Brunner, S., Herndler-Brandstetter, D., Arnold, C.R., Wiegers, G.J., Villunger, A., Hackl, M., Grillari, J., Moreno-Villanueva, M., Bürkle, A., Grubeck-Loebenstein, B. Upregulation of miR-24 is associated with a decreased DNA damage response upon etoposide treatment in highly differentiated CD8 + T cells sensitizing them to apoptotic cell death (2012) Aging Cell, 11 (4), pp. 579-587.

PubMed PMID: 22435726

Original research article on microRNAs that control proliferation of CHO cell lines, which are the most frequently mammalian cell line for recombinant therapeutic protein production.

Jadhav, V., Hackl, M., Hernandez Bort, J.A., Wieser, M., Harreither, E., Kunert, R., Borth, N., Grillari, J. A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells (2012) Biotechnology and Bioengineering, 109 (6), pp. 1376-1385.

PubMed PMID: 22407745

Bioinformatic analysis of microRNA sequences and genomic organization in CHO cell lines.

Hackl, M., Jadhav, V., Jakobi, T., Rupp, O., Brinkrolf, K., Goesmann, A., Pühler, A., Noll, T., Borth, N., Grillari, J. Computational identification of microRNA gene loci and precursor microRNA sequences in CHO cell lines (2012) Journal of Biotechnology, 158 (3), pp. 151-155

PubMed PMID: 22306111

Original research article describing  the changes in microRNA expression in vitro and ex vivo during aging.

Hackl, M., Brunner, S., Fortschegger, K., Schreiner, C., Micutkova, L., Mück, C., Laschober, G.T., Lepperdinger, G., Sampson, N., Berger, P., Herndler-Brandstetter, D., Wieser, M., Kühnel, H., Strasser, A., Rinnerthaler, M., Breitenbach, M., Mildner, M., Eckhart, L., Tschachler, E., Trost, A., Bauer, J.W., Papak, C., Trajanoski, Z., Scheideler, M., Grillari-Voglauer, R., Grubeck-Loebenstein, B., Jansen-Dürr, P., Grillari, J. miR-17, miR-19b, miR-20a, and miR-106a are down-regulated in human aging (2010) Aging Cell, 9 (2), pp. 291-296.

Opinion article on the potential of microRNAs for engineering the phenotype of cells to better fit to the needs in modern biotechnological processes.

Müller, D., Katinger, H., Grillari, J. MicroRNAs as targets for engineering of CHO cell factories (2008) Trends in Biotechnology, 26 (7), pp. 359-365.

Lepperdinger, G., Berger, P., Breitenbach, M., Frohlich, K.-U., Grillari, J., Grubeck-Loebenstein, B., Madeo, F., Minois, N., Zwerschke, W., Jansen-Durr, P. The use of genetically engineered model systems for research on human aging (2008) Frontiers in Bioscience, 13 (18), pp. 7022-7031.