Combining laser microdissection and microRNA expression profiling to unmask microRNA signatures in complex tissues
We want to draw your attention to the latest publication with our partner Vivomicx. Together we have developed a combination of laser microdissection (LMD) and high quality qPCR, which enables the quantitative analysis of cell-type specific RNA expression in complex tissues.
Using this protocol TAmiRNA & Vivomicx have been able to demonstrate:
the profound difference of RNA expression between whole tumor and subsets of cells within the tumor and
the large heterogeneity in RNA expression between colorectal* tumor compartments such as tumor, stroma and tumor endothelial cells.
* Note: the technology can be utilized for almost any tissue sample.
Our new approch allows to:
Discover differential expression patterns between “healthy” and “diseased” tissues on the cellular level;
Characterize localization of lead drug candidates on the single cell level;
Determine treatment effects on complex tissues at high resolution;
Gain a better understanding of a drug’s mode of action and of possible undesired side effects;
In the case of nucleic acid therapeutics: monitor localization of the therapeutic at high resolution.
Besides studying mRNA expression, we are particularly skilled in expression analysis of non-coding RNAs (microRNA and other ncRNAs). Vivomicx have massive experience with kidney tissue.