In this white paper, TAmiRNA and RoosterBio present a comprehensive small RNA-sequencing–based characterization of mesenchymal stem cell (MSC)–derived exosomes produced using RoosterBio’s standardized, xeno-free manufacturing platform. The study was designed to assess both the analytical robustness of TAmiRNA’s miND® small RNA-sequencing workflow and the biological relevance of microRNA cargo profiling as an advanced “omics-based” characterization strategy for therapeutic extracellular vesicles (EVs).
Using technical replicates and independent EV vials, we demonstrate that small RNA-sequencing performed with the miND® workflow yields highly reproducible and quantitatively consistent microRNA profiles, confirming the robustness of the analytical pipeline and the stability of RoosterBio’s EV products. In addition, comparative profiling of EVs derived from umbilical cord, bone marrow, and adipose tissue MSCs revealed distinct and tissue-specific microRNA signatures.
These source-dependent microRNA patterns are consistent with known biological functions of MSCs and suggest a potential link between EV microRNA cargo, mechanism of action, and therapeutic potency. Together, the results highlight the value of comprehensive, quantitative RNA profiling as a powerful tool for EV characterization, potency-relevant biomarker discovery, and CMC-aligned analytical development.
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