Sepsis drives profound microvascular injury, particularly in the kidney. But how do microRNAs (miRNAs) - the small regulators of gene expression - respond within distinct vascular compartments during this insult? A new study by Luxen et al.  published in Scientific Reports on October 2nd 2025 takes on this question using a clever methodological combination: laser microdissection (LMD) of microvascular subregions (arterioles, glomeruli, capillaries, postcapillary venules) followed by small RNA sequencing using TAmiRNA´s miND® workflow for highly sensitive and quantitative microRNA analysis. Why this approach matters Traditional microRNA profiling of whole tissues obscures spatial heterogeneity. With LMD, the authors could precisely isolate defined microvascular zones from kidney sections. Even with the tiny RNA yields from these isolated compartments, [...]