miND® Spike-In fulfills two primary purposes:
1. Quality control: confirm the dynamic range and quantitativeness of your small RNA sequencing experiments.
2. Absolute normalization: convert read counts to copies/µl total RNA and reduce bias originating from variation in RNA composition between samples.
miND® Spike-In design and usability:
miND® Spike-Ins are a proprietary oligonucleotide mix with unique design features (Lutzmayer et al.). They are added to your total RNA samples prior to microRNA or small RNA sequencing analysis. The spike-in sequence composition and concentration range have been optimized to be compatible with almost any species as well as a broad range of sample types including biofluids (serum, plasma, urine, CSF, synovial fluid), cells, tissues, extracellular vesicles, and non-vesicular fractions (Khamina et al.).