small RNA sequencing
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Watch this video of our user-friendly and interactive NGS results report (miND)

small RNA sequencing services

For untargeted genome-wide analysis of microRNAs and other small RNAs, next-generation sequencing (NGS) is the platform of choice.

At TAmiRNA we are fully dedicated to microRNA and small RNA NGS analysis. As we mainly work with extracellular RNA in biomarker research, we have long-lasting experience with very low-input RNA samples such as:

Our in-house developed miND® spike-ins enable absolute quantification and ensure the highest data quality, while RNA input can be reduced down to 1 ng total RNA or less.

Our NGS services go hand-in-hand with our

Quantitative NGS analysis of

  • microRNAs,
  • tRNAs (new!),
  • and other small ncRNAs

in any sample type and species. Click to receive a personalized quote for your project.

small RNA sequencing
small RNA sequencing

tRNA analysis now available

benefits

What we offer: our service includes total RNA extraction, small RNA sequencing library preparation, next-generation sequencing, data analysis, and reporting. Depending on customer experience we can focus on individual tasks or provide a full service.

Our promise: our small RNA sequencing workflow has undergone analytical validation. With best-in-class precision and accuracy, we can ensure the highest data quality. By using TAmiRNAs miND spike-ins we can transform relative counts into absolute concentrations (molecules/µL of input RNA).

Our experience: we have conducted small RNA sequencing analysis with samples from the following organisms: human, non-human primates, mouse, rat, hamster (CHO), pig, cow, horse, chicken, dogs, as well as C. elegans and drosophila. For other organisms data analysis can be implemented upon request. Browse our published reference projects (below) for more information on previous projects.

Your data: we provide interactive reports (see video above) that enable you to access and download your data so that it can be directly integrated into reports, publications, and presentations.

Your flexibility: we offer MiSeq, iSeq, NextSeq550, NextSeq1000/2000 and the NovaSeq. Thus, we manage to find a cost-effective solution for small and large projects.

sample requirements

Cells: we can go as low as 10-50 cells

Biofluids: serum, plasma, urine, saliva, CSF, milk, synovial fluid. The standard input is 100-200 µl but can be optimized to meet your restrictions.

Extracellular vesicles: characterization of EV RNA cargo can be performed by NGS or RT-qPCR. Due to the heterogeneity of methods that are used for EV purification, we prefer to discuss your project individually.

small RNA sequencing

Testimonials

“”I worked with Matthias Hackl and his TAmiRNA team for two projects. I was very happy with my results and found Matthias and his team very helpful and accommodating of our timeline for assay completion. Matthias provided invaluable assistance in the interpretation of the microRNA results. I highly recommend using TAmiRNA services for microRNA analysis.”.

Dr. Debra Bemben, Professor of Health and Exercise Science, Department of Health and Exercise Science, University of Oklahoma, OK, USA

“Since our first contact in July 2016, many projects have been successfully conducted with TAmiRNA, leading to peer-reviewed articles and novel findings on biomarkers. This achievement has been possible because of the expertise and support of TAmiRNA together with the speed of the service.” .

Dr. Bertrand Grenier and Dr. Veronika Nagl, BIOMIN Holding GmbH, Research Center, Tulln, Austria

“They have helped us getting the best from out data analysis and interpretation. Their flexibility and experience makes them an excellent partner of choice”.

Dr. Attila Kiss and Prof. Podesser, Center for Biomedical Research Medical University of Vienna
testing service
small RNA sequencing services