RNA profiling in complex tissues
RNA profiling in complex tissues

Cell-type specific and space-resolved RNA profiling in complex tissues

Healthy and malignant tissues are composed of multiple cell types: tumor and stromal cells, microvasculature and other specialized cell types (e.g., Tumor Infiltrating Lymphocytes). Therefore, the analysis of whole tumors results in a mixture of signals with unclear origin. Deconvolution of tissue heterogeneity and sensitive analytical methods are essential to obtain interpretable results from tissue analysis.

TAmiRNA and Dutch company Vivomicx have combined their respective technologies of high sensitivity gene expression profiling (RT-qPCR and smallRNAseq/RNAseq) and laser microdissection to enable cell-type specific and space-resolved RNA expression analysis in complex tissues.

This technology provides more accurate information on cellular states during onset and progression of diseases to accelerate drug and biomarker discovery.

LMD services
LMD services

benefits

Space-resolved and cell-type-specific gene expression analysis provide deep insights into disease mechanisms.

The high signal-to-noise ratio of space-resolved gene expression analysis enables the identification of novel drug and biomarker candidates.

The effect of drug-target engagement can be monitored in situ at the level of individual cell types, providing accurate insights into drug mode-of-action.

The combination of laser microdissection (LMD) and high sensitivity gene expression profiling can be applied to almost any biological tissue sample.

Every project is different and has specific requirements. Experts at Vivomicx and TAmiRNA have a combined experience of >15 years and are committed to finding customer-tailored solutions.

sample requirements

Laser microdissection requires preserved tissues such as fresh frozen or fixated tissue.

We acknowledge that every LMD project is different. Our technical experts will discuss sample requirements at the beginning of each project and pilot experiments can be performed to optimize performance.

RNA profiling in complex tissues
RNA profiling in complex tissues
RNA profiling in complex tissues